To improve the efficacy of treatment of drug-resistant ovarian cancer, the human epithelial ovarian cancer cell line SKOV3 and multidrug-resistant(mdr) phenotype SKVCR cells were exposed to different dose of radiation (0 Gy, 2 Gy, 4 Gy, 6 Gy, 8 Gy) or different radiation formulae (0 Gy, 1 Gy×2×5, 2 Gy×5, 10 Gy×1) with or without 3MA(autophagy Inhibitor via PI3K regulation)and ZVAD (apoptosis Inhibitor via caspase-3 regulation), the changes of cell viability, apoptosis, autophagy and cell cycle were analyzed in this study. This evidence concerns the gene CASP3 and ovarian cancer.