In the present study, we compared B7-H3 expression in normal pancreas andpancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression onthe human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference.B7-H3 expression in pancreatic specimens was determined by enzyme-linked immunosorbentassay (ELISA). Here, CD276 is linked to familial pancreatic carcinoma.