To evaluate alternative brain tumor targets, HB1.F3.eGFP NSCs were presented with established human glioma lines (U251.dsRed, U87, D566) and a series of patient-derived brain tumor lines [30] expanded under stem cell culture conditions (PBT006, PBT022, PBT024) or subcutaneously for 4 or 14 passages prior to stem cell medium culture (PBT003 scp4, PBT017 scp4, PBT017 scp14). This evidence concerns the gene CTDSPL2 and central nervous system cancer.