TNFSF10 and hepatocellular carcinoma: After single cell suspension isolation, primary HCC cells were cultured overnight for recovery from stress caused by cells isolation, then cells with high viability (90%–95%) were treated for 24 h with SM-164 (0.1 μM) alone, APO2L/TRAIL (100 ng/ml) alone, and their combination, and analyzed for viability.