To lend further support to our proposed mechanistic model that exposure of the CD4 binding site for improved CD4 binding and infection of CD4low cells is an early step in coreceptor switching, we generated viruses pseudotyped with CCR5-using Envs amplified over time from two additional SHIVSF162P3N-infected RPs with coreceptor switch (DE86, DG08). The gene discussed is CCR5; the disease is infection.