Univariate analysis and multivariate analysis in the current study showed that independent predictors of tumor recurrence and poor LT outcome for HCC patients with HBV-associated cirrhosis were tumor size >7.5 cm, tumor number >1, the presence of vascular and lymph node invasion, and pre-LT serum AFP levels ≥1000 ng/ml, and AST levels ≥120 IU/L. The gene discussed is AFP; the disease is Cirrhosis.