Multivariate analysis showed that independent predictors of poor recurrence-free survival were tumor size >7.5 cm (P = 0.001, HR = 3.309; CI 1.607–6.814), a tumor number >1 (P = 0.005, HR = 2.154; CI 1.260–3.682), the presence of vascular invasion (P = 0.001, HR = 2.788; CI 1.496–5.196), and a pre-LT serum AFP level ≥1000 ng/ml (P = 0.009, HR = 2.094; CI 1.200–3.653). The gene discussed is AFP; the disease is neoplasm.