To further investigate the effect of IGFBP-3 in the process of Nimotuzumab modulating of ESCC cells radiosensitivity, the expression levels of IGFBP-3 in KYSE30 cell line were first knocked down by a specific shRNA targeting IGFBP-3 gene (Figure 3b). This evidence concerns the gene IGFBP3 and esophageal squamous cell carcinoma.