To evaluate the biological functions of miR-30d during the progression of PCa, we inhibited miR-30d expression in PC3 (TP53 deficient cells) and LNCaP (TP53 wild-type [WT] cells) by transfecting antisense miR-30d oligonucleotides (Anti-miR-30d) (Figure 2A). Here, TP53 is linked to posterior cortical atrophy.