PHGDH and neoplasm: The mouse brain section staining confirmed that both the tumor size and invasion were dramatically inhibited after PHGDH silencing (Supplementary Tables 3 and 4) and a two-way ANOVA analysis of tumor diameter or invasion lesion numbers with respect to cell line (U87 versus U251) and shRNA expression was shown the results were significant (Fig. 5a, b, c).