To evaluate the potential effect of dovitinib at pharmacological concentration on the activation of RTK signaling pathways in vitro, we first examined the expression and activation of VEGFR, FGFR, PDGFR, Flt-3, and c-KIT in HCC cell lines and endothelial cell lines by immunoblotting. This evidence concerns the gene KDR and hepatocellular carcinoma.