The 2-DE analysis revealed 29 differentially expressed proteins between tumor and margin samples (Student’s t test p<0.05), most also found by 1-DE approach (except actin cytoplasmic 2, carbonic anhydrase 3, myoglobin, myosin light chain 3, superoxide dismutase [Cu-Zn] and troponin T slow skeletal muscle), and with an identical or similar expression pattern. This evidence concerns the gene ACTG1 and neoplasm.