Given the known role of Slug in inhibitting apoptotisis by regulating the Bcl-2, Bax expression and the transactivation of Puma in some tumours [14]; [15], the Bcl-2, Bax, and PUMA mRNA were assayed by RT-PCR (Fig. 7A) and the proteins were identified by western blot (Fig. 7C). Here, BBC3 is linked to neoplasm.