In nicotine infused CaMKIV +/+ mice, mecamylamine precipitated significant anxiety-related behavior [F(2,24) = 10.06, p<0.001], indicated by a decrease in the amount of time spent in the open arms of the plus maze (Figure 5A), increased somatic signs [F(2,24) = 120.16, p<0.0001; Figure 5B], and a significant hyperalgesia response [F(2,24) = 10.29, p<0.001], indicated by a decreased latency in the hot plate test (Figure 5C). The gene discussed is CAMK4; the disease is Anxiety.