Since emerging evidence appears to suggest that hCG may also exhibit angiogenic [14] and pro-invasive [15] activity, sera from non-immunized and immunized TG mice were evaluated for the ability to induce the transcription and expression of vascular endothelial growth factor (VEGF), KC (murine IL-8) as well as of matrix mellatoprotease (MMP)-9 from tumor cells. This evidence concerns the gene CALCA and neoplasm.