CA IX expression was visualized with a fluorescent CA IX antibody applied to ex vivo tissue slices, tumor hypoxic regions were visualized by i.v. administration of pimonidazole (HypoxyProbe) followed by ex vivo staining with a fluorescent antibody, and non hypoxic regions were identified by injection of Hoechst stain (a blood perfusion marker) 5 minutes prior to sacrifice, all 24 hours post injection of HS680 or control. The gene discussed is CA9; the disease is neoplasm.