Given the finding that the PI3K complex may be a key signaling network orchestrator of colorectal cancer metastasis, therapeutics targeting PI3K/AKT or the downstream mTOR complex [17] might be rational, and within this frame of reference, the ex vivo phosphosubstrate technology could show useful in developing the required biomarkers of signaling pathway druggability. Here, AKT1 is linked to colorectal cancer.