Zhang et al. evaluated the methylation status of 11 candidate genes (DAPK1, IGSF4, MLH1, HIC1, RARB, p14, TP73, MGMT, RASSF1, APC, and CDKN2A) in a set of 172 anal biopsies, which is the only published analysis of DNA methylation in anal cancer [9]. This evidence concerns the gene RARB and anal carcinoma.