This is the first study to our knowledge that provides data from MZT to trace plausible mechanistic pathways from molecular events (i.e. DNA methylation of CpG sites) in specific genes (i.e. FOXP3 and IFNγ) to transcript, to protein, to cellular changes and then to clinical outcomes and immune function (specifically, total IgE levels and tetanus response). This evidence concerns the gene IGHE and tetanus.