As shown in Figure 7A, the culture of MCF-7 cells under these conditions resulted in the formation of mammospheres that were then trypsinized, disaggregated and analyzed by flow cytometry showing an enrichment in CD44+/CD24low/- cells, a phenotypic characteristic that is considered a marker of CSCs in breast tumors [23-25]. The gene discussed is CD44; the disease is breast neoplasm.