NES and nasopharyngeal carcinoma: We used 5 μm-thick paraffin brain sections to check the survival and differentiation of injected cells by immunostaining with antibodies for NPC marker Nestin and differentiation markers for both neurons and astrocytes, together with human species-specific antibody for Vimentin (a marker for NPC and radial glia) to show the injected human NPC[25].