The Ag-intensities among Mf-positive persons were significantly higher in the entire study area (24950 units) as well as in the ‘transmission hotspots’(19953 units) compared to that among Mf-negatives (16.9 for the entire study area and 19.5 units for the ‘transmission hotspots’) indicating that a large number of infections are required to become microfilaraemic. The gene discussed is RENBP; the disease is infection.