However, pretreating IR-treated mice with the ChAT inhibitor HC-3 before IR removed this myocardial infarction sparing effect, as the ratio of the infarcted area returned to a level comparable with that of the control mice at 0.56±0.03 (vs. IR; P<0.05, n = 7). The gene discussed is CHAT; the disease is myocardial infarction.