HCMV and US28 modulation of intracellular Ca++ was also plotted as raw fluorescence (Figure S2), demonstrating that HCMV infection itself causes an increase in intracellular calcium likely due to UL37x1 as reported by Shenk and colleagues [49] and that US28 can cause a further, but more transient change in intracellular calcium that requires the addition of extracellular agonists such as RANTES. Here, CCL5 is linked to cytomegalovirus infection.