CD14 and tuberculosis: To determine the distribution and number of tetramer-bound and antigen-specific CD14+ macrophages in local TB lesions, fresh-frozen, 8-μm-thick sections of lung and lymph node from active TB patients were probed using the anti-MTB antibody and TCR tetramer or the anti-CD14 antibody and TCR tetramer, respectively, followed by nuclear staining with 4′, 6-diamidino-2-phenylindole (DAPI) and observation with confocal laser-scanning microscopy.