For detection cancer in ovarian biopsy on all stages including early one of the most perspective set from analyzed genes included 8 biomarkers: NKIRAS1/RPL15, THRB, RBPS3 (CTDSPL), IQSEC1, NBEAL2, ZIC4, LOC285205 and FOXP1. If methylation/deletion was found in two or more of these biomarkers then sample would be recognized as cancer. This evidence concerns the gene ZIC4 and cancer.