EN1 and ovarian cancer: Ten of the most well-known methylated genes in ovarian cancer were selected in Montavon et al., 2012 study: BRCA1, CDH1, DLEC1, EN1, GATA4, GATA5, HOXa9, HSULF1; RASSF1A and SFN. Although some of them showed very high frequency of methylation in HGSOC (e.g., SFN 100%) and HOXa9 and EN1 showed sensitivity of 98.8% and specificity of 91.7%, none of the markers after correction for multiple testing, gene methylation was not significantly associated with any clinico pathological characteristics including discrimination of Stages I + II from Stages III + IV [6].