Western blot analysis showed that VEGF expression was suppressed in Ad5-Eag1-shRNA group compared with Ad5-Control-shRNA group or saline group (Figure 3C), indicating that the anti-tumor effects of Eag1 silencing on osteosarcoma are related to decreased angiogenesis and reduced VEGF expression. The gene discussed is KCNH1; the disease is osteosarcoma.