First, using insulin pellets we partially attenuated the hyperglycaemia in TAM-treated Glis3fl/fl/Pdx1CreERT+ mice to <300 mg/dl, but immunoreactive insulin was still greatly reduced in the islets of these mice, whilst the rate of beta cell apoptosis was still significantly higher than that in the vehicle-treated control mice. Here, INS is linked to Hyperglycemia.