Also a double staining for VEGF-C with macrophages (ED-1) showed the large majority (>95%) of them are not positive for this growth factor, suggesting that ED-1 positive cells, at least in our model, are not the main cells for secreting VEGF-C, which is in line with recent report in unilateral ureteral obstruction (UUO) model [22]. Here, EDA is linked to Ureteral obstruction.