In the current study, established ovarian cancer cell lines, primary short-term cultured cell lines and fresh ovarian cancer cells derived from ascites and solid tumor specimens were evaluated for HER2 expression utilizing various detection methods, including quantitative PCR (q-PCR), western blot analysis and flow cytometry, and expression levels were compared to corresponding levels in normal ovarian surface epithelium cells. Here, ERBB2 is linked to ovarian carcinoma.