The validation of the Gb3 status of the tumor model and the tumor vessel were obtained by the staining for Alexa 568-conjugated 3E2 (red) and Alexa 488-conjugated CD31 (green) in NXS2 metastases frozen sections, which revealed a colocalization of Gb3 and endothelial marker as shown by the yellow staining on the merged image (Fig. 6A). Here, PECAM1 is linked to neoplasm.