To optimize VNS parameters, in the present study, we designed, for the first time, 6 different parameter sets for VNS in rats with AMI by UD method and examined their effects on conventional MI indexes such as serum LDH, CK and cTnT, and infarction size as well as newly developed index, serum level of TNF-α. Here, TNNT2 is linked to infarction.