Furthermore, in order to determine the cellular specific effect of Dph3 on tumor metastasis, we transfected human colon cancer cell HCT116, human ovary cancer cell A2780 and human skin cancer cell A431 with siDph3 plasmid and siCTL plasmid, followed by examination of the migration of these cell lines with transwell assay or wound healing. This evidence concerns the gene DPH3 and skin neoplasm.