To indentify whether the specific small hairpin RNA (shRNA) targeting OCT4 (OCT4-shRNA), Survivin (Sur-shRNA), or double shRNAs (Dual-shRNA) targeting both OCT4 and Survivin, influenced esophageal cancer cell proliferation, MTT assay was performed to detect cell viability. Here, BIRC5 is linked to esophageal cancer.