SNAI1 and hepatocellular carcinoma: Analysis of the mechanism underlying the effect of GLI1 on HCC cell growth showed that SNAI1, a target of this transcription factor [16], [17] was significantly increased in Huh7 cells overexpressing GLI1 at the mRNA (P = 0.0022; Figure 3A) and protein levels, as confirmed by immunofluorescence (Figure 3B, upper right panel) and Western immunoblotting (Figure 3B, lower right panel).