GLI1 and hepatocellular carcinoma: To determine whether ectopic expression of GLI1 increases HCC cell migration, we used the wound-healing assay and showed that the migration rate of Huh7-GLI1 cells was significantly higher than that of Huh7 Vector cells at both 24 and 48 hours after scratching (P = 0.005 and P = 0.004, respectively; Figure 1C).