Since 4 h co-culture of aATC with tumor cells showed surface staining of IFN-γ on tumor cells, we sought to determine the specificity of IFN-γ binding by adding 10 ng/ml recombinant human IFN-γ protein alone or with anti-IFN-γ antibody (10 μg/ml) in L3.6pl or MiaPaCa-2 cells with or without AT-101 for 1 h followed by washing and surface staining with anti-IFN-γ-PE. This evidence concerns the gene IFNG and neoplasm.