PPARG and obesity due to melanocortin 4 receptor deficiency: Contrary to the results of the in vitro assay in this study, Kuroda et al found that these constituents stimulate 3T3-L1 preadipocyte differentiation through PPARγ activation, indicating that unknown compounds in SAE besides dehydrodieugenol and dehydrodieugenol B may inhibit PPARγ activation and exert anti-obesity effects through regulation of adipogenic-related genes.