To determine whether the AURKA inhibitor MLN8237 inhibits the activation of AURKA phosphorylation on threonine-288 (T288) in melanoma cells, we treated Hs294T cells with MLN8237 for 3 days and performed Western blot analysis for phospho-AURKA (T-288) or phospho-AURKB (T-232). The gene discussed is AURKA; the disease is melanoma.