IL6 and melanoma: We then further tested the levels of IL-6 and IL-8 by ELISA in four melanoma cell lines (Hs294T, SK-Mel-2, SK-Mel-5 and SK-Mel-28) treated with MLN8237 or vehicle and confirmed that both IL-6 (Supporting Information Fig S11A) and IL-8 (Supporting Information Fig S11B) were increased following MLN8237 treatment.