NANOG and head and neck squamous cell carcinoma: To further verify the stem cell phenotype of the JLO-1 culture, a qPCR was performed to evaluate the expression of aldehyde dehydrogenase class-1A1 (ALDH1A1) and the transcription factors Oct-4 and Nanog in JLO-1 relative to a HNSCC cell line, UMSCC-10B, cultured under standard conditions.