GNRH1 and breast carcinoma: MDA cells transfected with the GnRHR (WT and DesK191) cDNAs, specifically bound 125I]-Buserelin and produced higher levels of the second messenger IP in response to the GnRH analog than untransfected cells, overcoming the problem related to the low naturally expressed GnRHR levels in breast cancer cell lines [50,51].