AKT1 and hepatocellular carcinoma: Because we did not observe an increase in the phosphorylation of AKT at S473 in HCC cells after treatment with a higher concentration of 100 nM RAD001 at 24 hours (Figure 1B), we performed a more detailed time kinetic from 3 up to 72 hour (Figure 1C and D, Additional file1: Figure S1).