Conversely, the percentage of splenic Th17 cells (CD4+ IL-17A+, Figure 4B) was elevated within both the HF and LF diet groups following the induction of colitis (within dietary groups vehicle control versus TNBS, P<0.05), which was not apparent in the HF-FO group (vehicle control versus TNBS-treated, P = 0.66). This evidence concerns the gene CD4 and colitis.