GHRL and obesity due to melanocortin 4 receptor deficiency: Thus, some studies evaluated pharmacological approaches to block or neutralize either ghrelin or its receptor under diet-induced obesity and how these methods ameliorate obesity condition by reducing appetite or food intake, and ultimately inducing weight loss (Wortley et al., 2005; Schellekens et al., 2010; Briggs and Andrews, 2011).