At an acute phase of toxoplasmosis, the T.g.HSP70 gene vaccine also limited the copy number of T. gondii in the mesenteric LN of WT, TLR2-deficient, and TRIF-deficient mice, but for neither TLR4-deficient nor MyD88-deficient mice, indicating the involvement of TLR4 in the vaccine effect at an acute phase of infection. Here, TLR2 is linked to toxoplasmosis.