In view of the bipartite NF-1 consensus sequence in the minimal −160/−90 CYP11A1 promoter, and sequence similarities between the minimal elements in the CYP17 and CYP11A1 promoters that conferred increased basal regulation in PCOS cells, studies were performed to investigate whether NF-1C2 coordinately regulates (i.e., represses) the CYP11A1 minimal promoter. Here, CYP11A1 is linked to polycystic ovary syndrome.