As shown as a comparison in Fig. 4A, co-transfection with empty pcDNA plasmid alone had no effect on −1676 CYP11A1/LUC promoter function, and both basal and forskolin-stimulated reporter function remained significantly augmented in PCOS theca cells, as compared to normal theca cells (a, b, P<0.01). Here, CYP11A1 is linked to polycystic ovary syndrome.