To determine if the −160/−90 region of the CYP11A1 promoter confers increased activity in PCOS theca cells, we transfected normal and PCOS theca cells with luciferase reporter constructs containing the −160/−90 bp region fused to a heterologous thymidine kinase promoter (Fig. 3A) or a luciferase construct containing the empty TK promoter (TK). Here, CYP11A1 is linked to polycystic ovary syndrome.