CYP11A1 and polycystic ovary syndrome: To test this possibility, we transfected PCOS theca cells with the CYP11A1 promoter construct containing the −160 to −90 sequence, −160/−90 TK (see Fig. 4C) and the empty control TK constructs with the pcDNA plasmid expressing NF-1C2 or the empty pcDNA plasmid.