In addition, to determine the extent to which the putative U-CRS consensus element confers both basal and cAMP dependent regulation in normal and PCOS theca cells, we constructed a CYP11A1 reporter construct containing the sequence between −1676 to −1540 bp fused to the minimal −90 CYP11A1 promoter construct (−1676Δ−1540/−90). The gene discussed is CYP11A1; the disease is polycystic ovary syndrome.