To examine the possibility that NF-1C2 coordinately regulates CYP11A1 gene expression in a manner similar to the CYP17A1 promoter, we performed studies to examine the effect of a human NF-1C2 pcDNA plasmid or an empty pcDNA plasmid on the full length −1676 CYP11A1 promoter construct in normal and PCOS theca cells. The gene discussed is CYP11A1; the disease is polycystic ovary syndrome.