VHL and hereditary clear cell renal cell carcinoma: In the PTEN and VHL-deficient renal cell carcinoma line 786-O, RAD001 and BEZ235 strongly synergized in vitro and reduced cell viability with excess inhibition over Loewe additivity when 2.5–67 nM BEZ235 were combined with RAD001 (Figure 3A and 3B).