In the PTEN and VHL-deficient renal cell carcinoma line 786-O, RAD001 and BEZ235 strongly synergized in vitro and reduced cell viability with excess inhibition over Loewe additivity when 2.5–67 nM BEZ235 were combined with RAD001 (Figure 3A and 3B). The gene discussed is VHL; the disease is renal cell carcinoma.