Our immunohistochemistry results further supported that HDAC6 was upregulated in cyst lining epithelia of Pkd1nl/nl mouse kidneys harvest at postnatal day 28 compared to that in the normal renal tubules of the age matched Pkd1 wild type mouse kidneys and the upregulated HDAC6 was localized in the cytosol, the site where HDAC6 was found in all the other cell types reported, of cystic epithelial cells (Fig. 1D). This evidence concerns the gene PKD1 and cyst.