In order to study X-chromosome inactivation in acute myeloid leukemia, here we describe a qauntitative nonradioactive method, analysing the polymorphisms of P55, IDS, and G6PD genes by RT-PCR and the generation of an allele specific restriction site in 200 Iranian healthy females and 45 female patients with AML aged 20–45 years before the treatment and during remission. Here, IDS is linked to acute myeloid leukemia.