MEG3 and hepatocellular carcinoma: Figure 4 A) demonstrate that the DNMT1 knockdown in HLE cells is able to induce re-expression of MEG3 accompanied by significant loss of DNA methylation at IG- and MEG3-DMR (Fig. 4 C), p<0.0001). The effectiveness of the siRNA-mediated knock-down of DNMT1 mRNA and protein in the HCC cell line HLE is shown in Figure S3.