DLK1 and hepatocellular carcinoma: However, Huang et al. [15] concentrate in their study of the DLK1-MEG3 locus in human HCC nearly exclusively on DLK1. While studying DLK1 expression using RT-PCR and immunohistochemistry in two independent and fairly large cohorts (82 and 88 cases, respectively) they measured the MEG3 RNA expression only in a small subset of samples (n = 10) and did not find any alteration.