With the objective to establish a 3D in vitro model for the label-free monitoring of tauopathy-related degeneration of neuronal cells, especially neuritic processes, that is highly reproducible and feasible for high-content screening systems, we generated SH-SY5Y cell lines stably expressing an N-terminal fused EGFP tau protein by lentiviral transduction. This evidence concerns the gene MAPT and tauopathy.