MAPT and Alzheimer disease: To recapitulate the AD-related tau pathology, we generated three SH-SY5Y cell lines that stably overexpress N-terminal EGFP-fused human tau (0N4R) variants: the wildtype (WT), the single mutated tau variant P301L and a novel fourfold mutated variant comprising the single point mutations ΔK280, P301L, V337M, R406W.