In males there was an interaction between MD and diet on GFAP mRNA levels (p<0.05), with HFD decreasing GFAP levels in controls and increase them in MD males, but these changes were not significant individually (CtCD: 100±9.5, CtHF: 77.9±5.1; MDCD: 67.3±6.0; MDHF: 81.5±9.8%). Here, GFAP is linked to Menkes disease.